Characterizing the role of microglia in cortical remyelination
Microglia, the resident macrophage of the brain, contribute to demyelination and remyelination in but less is known about their contributions in grey matter regions. We apply a toxin induced model of demyelination to define how microglia react to demyelination and then influence cortical remyelination.
Defining the reactive functional states of cortical astrocytes in demyelination and remyelination
Astrocytes are a highly heterogeneous, and functionally dynamic population of cells. Do reactive responses of astrocytes after demyelination aid or impair the ability to recover from myelin and damage and oligodendrocyte loss? We use a combination of transgenic mice, immunofluorescence, RNA in situ and molecular profiling to unravel how populations of astrocytes distributed of cortical space react to demyelination and influence remyelination.
Lab History:
The Orthmann-Murphy lab was launched in 2018. We use in vivo imaging, single cell RNA sequencing, and molecular biology techniques in mouse models of demyelination to better understand acquired and inherited myelin disorders.
Orthmann-Murphy Lab 